Most other stem cell labs continue to culture cells into Passage 3. This worsens and becomes shorter every time the cell culture is forced through another passage. Mesenchymal stem cell senescence is reported in cultures with population doubling time from 10-40. Population doubling time directly correlates with replicative senescence, loss of potency and genomic instability. Population doubling time refers to the number of times two-fold increase (doubling) of the cells in culture. In our partner laboratories as a standard, the most important parameter they use for determining clinical cell culture is using population doubling time. “A passage is when a portion of cells are transferred into new flasks to grow” Supportive therapies are also used to achieve the best clinical outcome. ![]() They are prepared within the cell laboratory and injected intravenously and into the spine treatment areas by experienced doctors and specialists. The added benefit for the patient is that no invasive surgery and trauma is required to harvest Umbilical Cord derived Stem Cells. Umbilical cord mesenchymal stem cells express a higher range and level of growth factors & specific cytokines when compared to adult mesenchymal stem cells. This is very important in facilitating "acceptance" of transplanted cells. Umbilical cord MSC’s lack class II HLA, whereas adult mesenchymal stem cells express these antigens. Umbilical cord MSCs appear to lack some immune properties compared with adult stem cells (the immune system doesn’t recognise them to induce an immune response). Our partner clinics and laboratories have visualised key differences between Umbilical Cord Mesenchymal Stem Cells and Adipose Fat / Bone Marrow derived sources: Have you noticed how a grazed knee or other injury tends to heal faster for a young child compared to an adult?Ĭord cells have the potential for increased therapeutic effect compared to autologous stem cells taken from an adult’s own body, particularly a body requiring repair because it has become ineffective at managing its own recovery and regeneration. This allows the administration of MSC preparations across MHC barriers without concern for immunological rejection or the need for immunosuppression, making Mesenchymal stem cells a universal stem cells source. Favourable Immune Status: Mesenchymal stem cells lack the co-stimulatory molecules of the B7 family that are required to initiate an immune response. Role as Regulatory Cells: Mesenchymal stem cells synthesize and secrete a variety of macromolecules that are known regulators of hematopoietic and bone-resorbing cells. Flexible Propagation: Mesenchymal stem cells can be grown and propagated in culture for extended periods, without losing differentiation potential. Ease of Growth in Culture: Advanced knowledge exists for how to grow Mesenchymal stem cells in culture, including protocols for isolation, expansion, and differentiation. These cell types include: fat (adipocytes), bone (osteoblasts), skin (dermal cells), nerve (neural cells), cartilage (chondrocytes), muscle (skeletal myocytes), tendons (tenocytes), marrow stroma, ligaments, and more. ![]() ![]() Diverse Differentiation Potential: Mesenchymal stem cells can form a variety of cell types in the laboratory, including those of both intra- and extra-mesenchymal lineage. Non-Controversial: Mesenchymal stem cells avoid the ethical issues of embryonic stem cells, as they can be derived from sources that include adult bone marrow, adipose tissue and donated umbilical cord blood. Well-Characterized: Mesenchymal stem cells are a well-characterized population of adult stem cells, with over 36,000 scientific articles published about them.
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